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Antibodies certain to the blocking peptide no more bind towards the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody compared to the antibody on your own, you can see which staining is distinct.
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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Blocking peptides are peptides that bind particularly towards the target antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.
Target Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation designs which contribute for the temporal coordination of each and every mitotic celebration. Cyclin E varieties a complex with and capabilities to be a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary and is also degraded as cells progress through S section.
Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was observed to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential part in advertising cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been explained. Two further splice variants read more were documented but specific nucleotide sequence info is just not nonetheless obtainable.
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